Phoresis; UV: ultraviolet; YFP: yellow fluorescent protein. Acknowledgements The authors would like to thank Prof. Dr. Martina Pohl and Dr. Achim Heck for helpful discussion. Author details 1 Institute of Molecular Enzyme Technology, Heinrich-Heine-University Duesseldorf, Juelich Research Center, Wilhelm-Johnen-Stra , D-52425 Juelich, Germany. 2AVT Biochemical Engineering, RWTH Aachen University, Worringerweg 1, D-52074 Aachen, Germany. 3Institute of Complex SystemsCellular Biophysics (ICS-2-dioxaborolane 3-Fluoro-5-iodobromobenzene Cyclobutylboronic acid 4), Juelich Research Center, Wilhelm-Johnen-Stra , D-52425 Juelich, Germany. Authors’ contributions TD developed the idea for the project and designed the experiments concerning the construction and characterization of the biosensor. JP designed and performed the experiments concerning the construction and characterization of the biosensor. MK and JB designed and performed the BioLector experiments. TG designed and performed the FLIM experiments. JP, MK, TG, TD and JB developed the method for data processing and analysis. TD and K-EJ wrote the paper. All authors read and approve the final manuscript. PubMed ID:https://www.ncbi.nlm.nih.gov/pubmed/11834444 Competing interests The authors declare competing financial interests. Parts of PubMed ID:https://www.ncbi.nlm.nih.gov/pubmed/8086425 the results described in this publication have Methyl 5-amino-2,4-difluorobenzoate been included into a patent application (Circolone F., Drepper T., Endres S. Heck A., Jaeger K.-E., Potzkei J. (2010); Patent number DE 10 2010 037 001). The oxygen biosensor FluBO will be commercialized by evocatal GmbH, Germany. Received: 16 December 2011 Accepted: 22 March 2012 Published: 22 March 2012 References 1. Aragon J, Fraisl P, Baes M, Carmeliet P: Oxygen sensors at the Fmoc-D-Asp-OtBu crossroad of metabolism. Cell Metab 2009, 9:11-22. 2. Ward JPT: Oxygen sensors in context. Biochim Biophys Acta 2008, 1777:1-14.13.14.15. 16. 17.18. 19.20.21.22. 23.24.25.26.27.28. 29. 30.Ernst JF, Tielker D: Responses to hypoxia in fungal pathogens. Cell Microbiol 2009, 11:183-190. Hassett DJ, Sutton MD, Schurr MJ, Herr AB, Caldwell CC, Matu JO: Pseudomonas aeruginosa hypoxic or anaerobic biofilm infections within cystic fibrosis airways. Trends Microbiol 2009, 17:130-138. Rustad TR, Sherrid AM, Minch KJ, Sherman DR: Hypoxia: a window into Mycobacterium tuberculosis latency. Cell Microbiol 2009, 11:1151-1159. Schobert M, Jahn D: Anaerobic physiology of Pseudomonas aeruginosa in the cystic fibrosis lung. Int J Med Microbiol 2011, 300:549-556. Schobert M, Tielen P: Contribution of oxygen-limiting conditions to persistent infection of Pseudomonas aeruginosa. Future Microbiol 2010, 5:603-621. Eltzschig HK, Carmeliet P: Hypoxia and inflammation. N Engl J Med 2011, 364:656-665. Brown JM: Exploiting the hypoxic cancer cell: mechanisms and therapeutic strategies. Mol Med Today 2000, 6:157-162. Brown JM: Tumor hypoxia in cancer therapy. Methods Enzymol 2007, 435:297-321. Gordan JD, Simon MC: Hypoxia-inducible factors: central regulators of the tumor phenotype. Curr Opin Genet Dev 2007, 17:71-77. Lu X, Kang Y: Hypoxia and hypoxia-inducible factors: master regulators of metastasis. Clin Cancer Res 2010, 16:5928-5935. Coates JD, Anderson RT: Emerging techniques for anaerobic bioremediation of contaminated environments. Trends Biotechnol 2000, 18:408-412. Karakashev D, Thomsen AB, Angelidaki I: Anaerobic biotechnological approaches for production of liquid energy carriers from biomass. Biotechnol Lett 2007, 29:1005-1012. L fler FE, Edwards EA: Harnessing microbial activities for environmental cleanup. Curr Opin Biotechnol 2006, 17:274-284. McKinlay JB, Harwood CS:.
Phoresis; UV: ultraviolet; YFP: yellow fluorescent protein. Acknowledgements The authors would
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